HuR, a protein that binds to specific mRNA subsets, is increasingly recognized as a pivotal posttranscriptional regulator of gene expression. Here, HuR was immunoprecipitated under conditions that preserved HuR-RNA interactions, and HuR-bound target mRNAs were identified by cDNA array hybridization.

Jun 18, 2020 The RNA-binding protein (RBP) Hu antigen R (HuR), also known as embryonic lethal abnormal vision-like protein 1 (ELAVL1), is a ubiquitously

We generated 2 stable U251 clones expressing short hairpin (sh) RNA directed to HuR (shHuR). A scrambled sequence was utilized to generate control (shControl) clones. Effective and specific knockdown of HuR was observed at the mRNA and protein levels (Supplementary Fig. S1). Human antigen R (HuR) is an RNA-binding protein that posttranscriptionally regulates many cancer-trait genes. CDC6, a central regulator of DNA replication, is regulated by HuR. In this study, we investigated the role of HuR in colorectal cancer tumorigenesis and oxaliplatin (L-OHP) resistance, as well as the underlying mechanisms involving CDC6. We detected increased HuR and CDC6 expression Coordinating such RNA regulons is often the responsibility of regulatory RBPs that have key roles in immunity like the polypyrimide track protein 1 (PTBP1), embryonic lethal abnormal vision like protein 1 (ELAVL1, also known as HuR), or the members of the zinc finger protein 36 family (ZFP36, ZFP36L1 and ZFP36L2; also known as TTP or Tis11-family of proteins). The RNA-binding protein HuR associates with the p53 mRNA, as reported previously, and with the NPM mRNA, computationally predicted to be a target of HuR. Here, we show that HuR binds the NPM and p53 3′-untranslated regions and stabilizes these mRNAs in polyamine-depleted intestinal epithelial cells.

Hur rna binding protein

Here the authors show that HuR represses adipogenesis in white and brown adipose tissue by stabilizing Insig1 and ELAV-like protein 1 or HuR is a protein that in humans is encoded by the ELAVL1 gene. The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. One of its best-known functions is to stabilize mRNAs in order to regulate gene expression. 2018-04-20 · METHODS AND RESULTS: Herein, we show that, as evidenced by ribonucleoprotein immunoprecipitation assay, RNA binding protein Hu antigen R/ELAV like RNA binding protein 1 (HuR/ELAVL1) and myocyte enhancer factor-2C (MEF2C) transcription factor mRNA are associated. HuR positively regulated transcription factor MEF2C mRNA expression by protecting its mRNA from degradation. 2021-03-16 · RNA binding protein, Hu Antigen R (HuR), is associated with myocyte enhancer factor‐2C (MEF 2C) mRNA, and the association protects MEF 2C mRNA from degradation.

All treatments on The RNA binding protein HuR determines the differential translation of autism-associated FoxP subfamily members in the developing neocortex The RNA-binding protein HuR is a negative regulator in adipogenesis Abstract. Human antigen R (HuR) is an essential regulator of RNA metabolism, but its function in metabolism remains Introduction.

The RNA-binding protein HuR associates with the p53 mRNA, as reported previously, and with the NPM mRNA, computationally predicted to be a target of HuR. Here, we show that HuR binds the NPM and p53 3′-untranslated regions and stabilizes these mRNAs in polyamine-depleted intestinal epithelial cells.

2018-04-20 · METHODS AND RESULTS: Herein, we show that, as evidenced by ribonucleoprotein immunoprecipitation assay, RNA binding protein Hu antigen R/ELAV like RNA binding protein 1 (HuR/ELAVL1) and myocyte enhancer factor-2C (MEF2C) transcription factor mRNA are associated. HuR positively regulated transcription factor MEF2C mRNA expression by protecting its mRNA from degradation. 2021-03-16 · RNA binding protein, Hu Antigen R (HuR), is associated with myocyte enhancer factor‐2C (MEF 2C) mRNA, and the association protects MEF 2C mRNA from degradation.

RNA Genomics Solution company empowering RNA Researchers around the to profile binding sites for RNA binding proteins, using enhanced crosslinking

import of proteins. KEY WORDS: HuR, RNA stability, Nuclear import, Retinoic acid, Cellular retinoic acid-binding protein, NFκB INTRODUCTION One of the best-characterized RNA-binding proteins in animals is HuR (encoded for by Elavl1), a ubiquitously-expressed member of the embryonic lethal abnormal vision (ELAV)/Hu family of RNA-binding proteins. Proteins of the Hu family of RNA-binding proteins, which comprises the ubiquitous HuR and the primarily neuronal proteins HuB, HuC, and HuD (1–5), are emerging as pivotal regulators of posttranscriptional gene expression.

Crossref, Medline, Google Scholar; Gallardo T, Shirley L, John GB, Castrillon DH (2007). Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre. Genesis 45, 413-417. Crossref, Medline, Google Scholar Growing evidence shows that cancer cells use mRNA-binding proteins and miRNAs to posttranscriptionally regulate signaling pathways to adapt to harsh tumor microenvironments. In ovarian cancer, cytoplasmic accumulation of mRNA-binding protein HuR (ELAVL1) is associated with poor prognosis. In this study, we observed high HuR expression in ovarian cancer cells compared with ovarian primary cells RNA-binding proteins represent an emerging class of proteins with a role in renal dysfunction.
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2012-06-01 FIGURE 6 RNase footprint analysis of HuR binding to ERBB-2 MT-URE/WT-331b target RNA. Lane labeling, protein concentrations, and RNase designations are identical to the gel shown in Fig. 5 except that the target RNA used is the ERBB-2 MT-URE/WT-331b target RNA. *, putative HuR binding site. 2014-08-27 ADAR : an RNA binding protein involved in RNA editing events.. The most extensively studied form of RNA editing involves the ADAR protein.

2011-12-27 · Several studies have linked the RNA-binding protein HuR, which increases mRNA stability, with malignant transformation.
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2011-08-05 · The RNA-binding protein Elavl1/HuR is essential for placental branching morphogenesis and embryonic development

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ubiquitously expressed mRNA-binding protein (mRBP) HuR(ELAVL1)isapostulatedstress-relevantproteinthat binds to adenylate uridylate (AU)–rich sites within regu-latory mRNA targets and regulates their expression via post-transcriptional mechanisms (12). These binding sites typically reside within 39–UTRs of target transcripts.

The RNA-binding protein HuR associates with the p53 mRNA, as reported previously, and with the NPM mRNA, computationally predicted to be a target of HuR. Here, we show that HuR binds the NPM and p53 3′-untranslated regions and stabilizes these mRNAs in polyamine-depleted intestinal epithelial cells. Albeit the suppression of mRNA translation may derive from eIF3a binding to the 5′-UTRs of target mRNAs, how eIF3a may accelerate mRNA translation remains unknown. In this study, we show that eIF3a up-regulates translation of Chk1 but not Chk2 mRNA by interacting with HuR, which binds directly to the 3′-UTR of Chk1 mRNA.